|
Title:
|
THREE-DIMENSIONAL VISUALIZATION AND AUTOMATED QUANTITATIVE ANALYSIS OF CULTURED NEURONS PLATED ON MULTI-ELECTRODE ARRAYS |
|
Author(s):
|
João Fernando Mari, José Hiroki Saito, Amanda Ferreira Neves, João Batista Destro-Filho, Celina Monteiro da Cruz Lotufo |
|
ISBN:
|
978-989-8533-06-7 |
|
Editors:
|
Hans Weghorn, Leonardo Azevedo and Pedro Isaías |
|
Year:
|
2011 |
|
Edition:
|
Single |
|
Keywords:
|
Multi-electrode arrays, confocal fluorescence microscopy, three-dimensional visualization, automated quantitative analysis, neural culture, polygonal mesh. |
|
Type:
|
Full Paper |
|
First Page:
|
179 |
|
Last Page:
|
186 |
|
Language:
|
English |
|
Cover:
|
|
|
Full Contents:
|
click to dowload 
|
|
Paper Abstract:
|
Multi-electrode arrays (MEAs) can stimulate and record extracellular electrical activities of an entire dissociated culture of neuron cells over long periods. Optical confocal fluorescence microscopy enables to visualize the culture state, and extract important quantitative parameters related to spatial distribution of the cells on the MEA. This work presents a system developed to generate polygonal 3D representations and perform a full-automated quantitative analysis of the MEA cultured cells, based on a biological model associated to the dorsal-root ganglia (DRG) of adult rats. Semi-automatic and manual classification steps are necessary for false-positive reduction, and the quantitative analysis is performed by automatically processing the remaining polygonal surfaces. Results point out that our proposition is capable of efficiently reducing false-positive reconstructions in an interactive way, and that quantitative analysis provides a simple description of the culture attached to the MEA. |
|
|
|
|
|
|
